Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts
Figure 3
Knockdown of JNK prohibited the upregulation of gallic acid-elicited p53 accumulation and apoptosis-related molecule expression. (a) MLFs were treated with control-siRNA or the indicated concentrations of JNK siRNA for 16 h. Cell lysates were analyzed by Western blot with antibodies against JNK. (b) MLFs were treated with control-siRNA or JNK siRNA in maintenance medium for 16 h followed by stimulation with 50 g/mL gallic acid for 1 h (for p53) or 24 h (for PUMA and Fas). Cell lysates were analyzed by Western blot with antibodies against p53, PUMA, and Fas. (c) MLFs were treated with control-siRNA or JNK siRNA in maintenance medium for 16 h followed by stimulation with 50 g/mL gallic acid for 24 h. The apoptotic cells were determined by TUNEL assay. Data were expressed as the mean SD from three independent experiments.
