Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts
Figure 4
Antioxidants and catalase inhibit gallic acid-mediated ROS production and apoptotic process. (a) MLFs were pretreated with 100 M ascorbic acid (Asc) or 3 mM N-acetylcysteine (NAC) for 30 min and then incubated with 50 g/mL gallic acid for another 1 h or 24 h. The levels of p-ATM, p-JNK, p53, PUMA, and Fas were detected by Western blot analysis. (b)-(c) MLFs were preincubated with catalase for 1 h and then treated with gallic acid for another 30 min for DCF-DA fluorescence analysis by flow cytometry or 24 h for apoptosis determination by TUNEL assay. (d) MLFs were preincubated with catalase for 1 h and then treated with gallic acid for another 1 h. The levels of p-ATM, p-JNK, p53, PUMA, and Fas were detected by Western blot analysis.
