Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts
Figure 5
Synergistic effects of ATM and JNK on gallic acid-induced MLF apoptosis. (a) MLFs were pretreated with SP600125 and/or KU55933 for 1 h and then incubated with 50 g/mL gallic acid for 24 h. The apoptotic cells were determined by TUNEL assay. Data were expressed as the mean SD from 3 independent experiments. (b) MLFs were pretreated with KU55933 (ATM kinase inhibitor) for 1 h and then incubated with 50 g/mL gallic acid for another 1 h. Cell lysates were analyzed by Western blot with antibodies against p-JNK. (c) MLFs were pretreated with SP600125 (JNK kinase inhibitor) for 1 h and then incubated with 50 g/mL gallic acid for another 1 h. Cell lysates were analyzed by Western blot with antibodies against p-ATM.
