Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 614501, 5 pages
http://dx.doi.org/10.1155/2013/614501
Research Article

Antimicrobial Activity of Lippia Species from the Brazilian Semiarid Region Traditionally Used as Antiseptic and Anti-Infective Agents

1Laboratório de Química de Produtos Naturais e Bioativos, Departamento de Ciências Exatas, Universidade Estadual de Feira de Santana, Avenida Transnordestina S/N, Bairro Novo Horizonte, Campus Universitário, 44036-900 Feira de Santana, BA, Brazil
2Laboratório de Microbiologia da Agroindústria, Universidade Estadual de Santa Cruz, Campus Soane Nazaré de Andrade, Km 16 Rodovia Ilhéus-Itabuna, 45662-900 Ilhéus, BA, Brazil
3Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Avenida Transnordestina S/N, Bairro Novo Horizonte, Campus Universitário, 44036-900 Feira de Santana, BA, Brazil

Received 15 July 2013; Accepted 7 August 2013

Academic Editor: Vincenzo De Feo

Copyright © 2013 Cristiana da Purificação Pinto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lippia origanoides Kunth, Lippia alnifolia Schauer, and Lippia thymoides Martius and Schauer are shrubs used in the traditional Brazilian medicine as antiseptics, as well as in the treatment of infectious diseases. This study was designed to investigate the antibacterial and antifungal activities of the methanolic extracts of these species, as new potential sources of antimicrobial drugs. The antimicrobial activity of methanolic extracts was investigated against resistant yeasts and bacteria by agar disk diffusion. Then, the MIC determination of the most active species and its fractions in hexane, dichloromethane, ethyl acetate, and water was performed. By the agar diffusion assay, all species were active against at least two microorganisms, giving evidence to support their use in the popular medicine. L. origanoides leaves exhibited the widest antimicrobial action, inhibiting the growth of two Gram-positive bacteria and two yeasts; this activity was also confirmed by the MIC evaluation. The fractionation of L. origanoides crude extracts improved the activity in spectrum and intensity. The results obtained in this study indicate that L. origanoides may be a promising alternative in the treatment of bacterial and fungal infections and in the seeking of new antimicrobial drugs.