Bees’ Honey Attenuation of Metanil-Yellow-Induced Hepatotoxicity in Rats
Table 2
Antioxidant parameters as well as AGE levels of rat liver homogenate. Group 1, control group; groups 2–4, rats treated with 50, 100, and 200 mg metanil yellow/kg body weight for 8 weeks, respectively; groups 5–7, rat groups treated with 50, 100, and 200 mg metanil yellow/kg body weight plus 2.5 g/kg bees’ honey daily for 8 weeks, respectively.
Group 1
Group 2
Group 3
Group 4
Group 5
Group 6
Group 7
MDA, nmol/mg protein
0.42 0.06
1.49 0.19a
5.1 0.85a
9.37 1.8a
0.85 0.02b
1.45 0.35a,c
1.9 0.92a,d
GSH, nmol/mg protein
101 5.8
72.5 1.6a
45.9 4.6a
26.7 3.8a
79.5 2.1
78.5 2.1a,c
80.2 1.3a,d
GST, nmol/mg protein
18.6 1.5
11.1 1.3a
6.29 0.39a
4.2 0.94a
14.76 0.86b
10.54 0.45a,c
10.3 0.87a,d
SOD, U/mg protein
15.8 ± 1.1
10.5 2.5a
9.6 2.1a
3.1 1.2a
14.25 1.1b
11.2 1.3a,c
10.97 1.5a,d
CAT, U/mg protein
5.95 ± 0.5
3.12 ± 0.2a
2.41 0.31a
1.1 0.11a
4.56 0.54b
4.29 0.23c
3.1 0.67a,d
GSH-Px, U/mg protein
4.95 ± 0.21
3.1 0.31a
2.05 0.12a
1.15 0.10a
4.67 0.65b
4.12 0.32c
3.87 0.56d
AGE, ng/mg protein
20.6 ± 4.5
26.2 5.5a
36.5 5.5a
48.7 8.5a
22.2 5.5b
28.2 5.5a,c
32.2 5.5a,d
All values were expressed as mean ± S.D. of triplicate tests ( = 10).
a < 0.05 versus group 1, b < 0.05 versus group 2, c < 0.05 versus group 3, d < 0.05 versus group 4.