Cytotoxic Activity of Six Samples of Brazilian Propolis on Sea Urchin (Lytechinus variegatus) Eggs

Table 1

Cytotoxic activity, (*) expressed as percent of affected sea urchin (Lytechinus variegatus) embryos, of methanol extract (MeOH) and hexane, chloroform (CHCl₃), ethyl acetate (EtOA), and methanol (MeOH) fractions at 32 µg mL⁻¹ (see text) from samples (**) of Brazilian propolis. No effect was observed in tests using ethanol (control).

Extract/fractions (µg/mL)			Samples
	A	B	C	D	E	F
MeOH extract	98.5 ± 2.1	97.3 ± 2.1	100 ± 0.0	99.3 ± 0.6	97.7 ± 3.2	97.3 ± 1.5
Fractions
Hexane	98.5 ± 2.1	64.7 ± 7.5	27.5 ± 0.7	5.0 ± 2.1	4.3 ± 2.1	6.3 ± 1.5
CHCl3	83.0 ± 1.4	83.3 ± 7.5	64.0 ± 4.2	92.5 ± 0.7	2.0 ± 1.0	15.0 ± 1.4
EtOAc	95.5 ± 2.1	98.0 ± 2.0	100 ± 0.0	100 ± 0.0	99.7 ± 0.6	100 ± 0.0
MeOH	3.5 ± 2.1	2.3 ± 1.2	3.3 ± 0.6	3.5 ± 0.7	4.7 ± 2.5	1.7 ± 1.2

∗Mean ± s.d.; **A–D: samples from Minas Gerais, E and F: samples from Paraná state.