Therapeutic Effects of Water Soluble Danshen Extracts on Atherosclerosis
Figure 1
The antioxidant, antimigratory, and antiproliferative effects of Danshen extract on PDGF-BB-treated vascular smooth muscle cells. Platelet-derived growth factor (PDGF)-BB (20 ng/mL) was treated for 24 h after Danshen extracts (DE) treatment at each concentration for 24 h. (a) Fluorescence activated cell sorting for PDGF-BB-induced intracellular reactive oxygen species in vascular smooth muscle cells (VSMCs). (b) Western blotting for manganese superoxide dismutase and hemoxygenase-1. DE (100 μg/mL) treated for 24 h before PDGF treatment. (c) Western blotting for proteins on Akt signaling pathway after treatment DE (100 μg/mL) for 24 h. (d) The wound-healing experiment with or without DE (100 μg/mL, 24 h). (e) Proliferation assessed by the MTT cell proliferation assay. Relative proliferation activities were expressed using untreated control cells as a standard. Results are expressed as the mean ± standard error; * versus vehicle treatment after PDGF-BB induction. (f) The effects of DE on cell cycle progression. DE: Danshen extract.
