(a) −Exendin-9
(b) +Exendin-9
(c) AUC
Figure 6: Effects of exendin-9 on the acute hypoglycemic effect of WES. Changes in plasma glucose concentration after a single oral dose of WES to high-fat-diet fed mice without (a) or with (b) a prior administration of Exendin-9, a GLP-1 receptor antagonist. Mice were fasted for 6 hr and blood samples collected at time 0. PBS (a) or 0.12 mg/kg BW Exendin-9 (b) were i.p. injected. Five minutes later, WES (3000 mg/kg BW) (S, SE) or vehicle (V, VE) containing equal amount of glucose as in WES (258 mg/kg BW) were administered orally and blood samples collected at the time points indicated. (c) area under curve of (a) and (b). Data are mean ± SD with for each group. * and ** denote significant difference compared to vehicle-treated mice analyzed by Student’s test.