Subamolide B Isolated from Medicinal Plant Cinnamomum subavenium Induces Cytotoxicity in Human Cutaneous Squamous Cell Carcinoma Cells through Mitochondrial and CHOP-Dependent Cell Death Pathways
Figure 1
Cytotoxic effect of subamolide B on human skin cancer cell lines. (a) Dose-dependent cytotoxicity of subamolide B. Human skin cancer cell lines, including A375 (melanoma), A-431 (epidermoid carcinoma), BCC-1/KMC (basal cell carcinoma) and SCC12 (cutaneous squamous cell carcinoma), normal human skin fibroblast cell lines CCD-966SK and WS1, and normal human lung fibroblast cell lines IMR-90, MRC-5, and WI-38 were treated with graded doses of subamolide B (0~20 μM), followed by cell viability determination via MTS assay as described in Section 2. (b) Dose-dependent repression of SCC12 colony formation by subamolide B. SCC 12 cells were treated with subamolide B (0~20 μM) for 24 h and then allowed to grow in drug-free media to form colonies. 14 days later, the extent of subamolide B-mediated repression of SCC12 colony formation was evaluated by the numbers of colonies grown on culture dishes. Shown here are the representative images from at least three independent experiments. (c) Quantitative analysis of subamolide B-mediated repression of SCC12 colony formation. The numbers of colonies shown in (b) were counted and the results were presented as the percentage of drug-treated groups compared to the drug-free controls. ***.
