Magnolol Ameliorates Ligature-Induced Periodontitis in Rats and Osteoclastogenesis: In Vivo and In Vitro Study

Figure 6

Effect of magnolol on RANKL-induced osteoclast differentiation. RAW 264.7 cells were cultured with the indicated dose of magnolol (2.5–20 μM) in the presence of RANKL (50 ng/mL) for 4 days. The photographs of TRAP-stained osteoclasts (a), the number of TRAP⁺ MNCs, and the TRAP activity (b) were examined (scale bar = 100 μm). Data are expressed as the mean ± SD (𝑛=5). *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 versus RANKL-treated group alone.

(a)

(b)