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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 641480, 18 pages
http://dx.doi.org/10.1155/2013/641480
Research Article

Evaluation of Mucoadhesive Gels with Propolis (EPP-AF) in Preclinical Treatment of Candidiasis Vulvovaginal Infection

1Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil
2Laboratório de Pesquisa, Desenvolvimento e Inovação, Apis Flora Indl. Coml. Ltda., 14020-670 Ribeirão Preto, SP, Brazil
3Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, 14040-903 Ribeirão Preto, SP, Brazil
4Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14040-040 Ribeirão Preto, SP, Brazil
5Laboratório Nacional de Ciência e Tecnologia do Bioetanol-CTBE, Caixa Postal 6170, 13083-970 Campinas, SP, Brazil

Received 29 March 2013; Revised 12 June 2013; Accepted 13 June 2013

Academic Editor: Wojciech Król

Copyright © 2013 Andresa Aparecida Berretta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. D. Sobel, “Vulvovaginal candidosis,” Lancet, vol. 369, no. 9577, pp. 1961–1971, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. P. L. Fidel Jr., “Vaginal candidiasis: review and role of local mucosal immunity,” AIDS Patient Care and STDs, vol. 12, no. 5, pp. 359–366, 1998. View at Google Scholar · View at Scopus
  3. J. R. Naglik, S. J. Challacombe, and B. Hube, “Candida albicans secreted aspartyl proteinases in virulence and pathogenesis,” Microbiology and Molecular Biology Reviews, vol. 67, no. 3, pp. 400–428, 2003. View at Publisher · View at Google Scholar · View at Scopus
  4. P. L. Fidel Jr., J. L. Cutright, and J. D. Sobel, “Efficacy of D0870 treatment of experimental Candida vaginitis,” Antimicrobial Agents and Chemotherapy, vol. 41, no. 7, pp. 1455–1459, 1997. View at Google Scholar · View at Scopus
  5. H. Lotz, H. Brunner, and S. Rupp, “Hyphae-specific cell wall proteins of Candida,” US2007/0196378A1, 2007.
  6. S. Perea, J. L. López-Ribot, W. R. Kirkpatrick et al., “Prevalence of molecular mechanisms of resistance to azole antifungal agents in Candida albicans strains displaying high-level fluconazole resistance isolated from human immunodeficiency virus-infected patients,” Antimicrobial Agents and Chemotherapy, vol. 45, no. 10, pp. 2676–2684, 2001. View at Publisher · View at Google Scholar · View at Scopus
  7. M. A. Pfaller and D. J. Diekema, “Rare and emerging opportunistic fungal pathogens: concern for resistance beyond Candida albicans and Aspergillus fumigatus,” Journal of Clinical Microbiology, vol. 42, no. 10, pp. 4419–4431, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. B. A. Rocha, M. R. Rodrigues, P. C. P. Bueno et al., “Preparation and thermal characterization of inclusion complex of Brazilian green propolis and hydroxypropyl-β-cyclodextrin: increased water solubility of the chemical constituents and antioxidant activity,” Journal of Thermal Analysis and Calorimetry, vol. 108, no. 1, pp. 87–94, 2012. View at Publisher · View at Google Scholar · View at Scopus
  9. N. Volpi and G. Bergonzini, “Analysis of flavonoids from propolis by on-line HPLC-electrospray mass spectrometry,” Journal of Pharmaceutical and Biomedical Analysis, vol. 42, no. 3, pp. 354–361, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. M. L. Bruschi, D. S. Jones, H. Panzeri, M. P. D. Gremião, O. De Freitas, and E. H. G. Lara, “Semisolid systems containing propolis for the treatment of periodontal disease: in vitro release kinetics, syringeability, rheological, textural, and mucoadhesive properties,” Journal of Pharmaceutical Sciences, vol. 96, no. 8, pp. 2074–2089, 2007. View at Publisher · View at Google Scholar · View at Scopus
  11. G. P. Andrews, T. P. Laverty, and D. S. Jones, “Mucoadhesive polymeric platforms for controlled drug delivery,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 71, no. 3, pp. 505–518, 2009. View at Publisher · View at Google Scholar · View at Scopus
  12. J. P. B. de Sousa, P. C. P. Bueno, L. E. Gregório et al., “A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography,” Journal of Separation Science, vol. 30, no. 16, pp. 2656–2665, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. A. A. Berretta, A. P. Nascimento, P. C. P. Bueno, M. M. D. O. L. Leite Vaz, and J. M. Marchetti, “Propolis standardized extract (EPP-AF), an innovative chemically and biologically reproducible pharmaceutical compound for treating wounds,” International Journal of Biological Sciences, vol. 8, no. 4, pp. 512–521, 2012. View at Publisher · View at Google Scholar · View at Scopus
  14. U. V. C. de Andrade, W. Hartmann, S. Funayama, S. M. de Alencar, and M. L. Masson, “Propolis obtained by means of alkaline hydrolysis and action on Staphylococcus aureus,” Ars Veterinaria, vol. 25, no. 3, pp. 151–154, 2009. View at Google Scholar
  15. J. L. Machado, A. K. M. Assunção, M. C. P. da Silva et al., “Brazilian green propolis: anti-inflammatory property by an immunomodulatory activity,” Evidence-Based Complementary and Alternative Medicine, vol. 2010, Article ID 157652, 10 pages, 2012. View at Publisher · View at Google Scholar
  16. A. M. Gillum, E. Y. H. Tsay, and D. R. Kirsch, “Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations,” Molecular and General Genetics, vol. 198, no. 1, pp. 179–182, 1984. View at Google Scholar · View at Scopus
  17. W. A. Fonzi and M. Y. Irwin, “Investigations—isogenic strain construction and gene mapping in Candida albicans,” Genetics, vol. 134, no. 3, pp. 717–728, 1993. View at Google Scholar · View at Scopus
  18. Clinical and Laboratory Standards Institute (CLSI), Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard, CLSI document M27-A3, 3rd edition, 2008.
  19. J. Yano and P. L. Fidel Jr., “Protocols for vaginal inoculation and sample collection in the experimental mouse model of Candida vaginitis,” Journal of Visualized Experiments, no. 58, article 3382, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. T. Urushisaki, T. Takemura, S. Tazawa et al., “Caffeoylquinic acids are major constituents with potent anti-influenza effects in brazilian green propolis water extract,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 254914, 7 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. S. P. Andrade, S. A. L. D. Moura, G. Negri et al., “Aqueous extract of Brazilian green propolis: primary components, evaluation of inflammation and wound healing by using subcutaneous implanted sponges,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 748283, 8 pages, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Matsui, S. Ebuchi, T. Fujise et al., “Strong antihyperglycemic effects of water-soluble fraction of Brazilian propolis and its bioactive constituent, 3,4,5-tri-O-caffeoylquinic acid,” Biological and Pharmaceutical Bulletin, vol. 27, no. 11, pp. 1797–1803, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. P. A. de Castro, M. Savoldi, D. Bonatto et al., “Molecular characterization of propolis-induced cell death in Saccharomyces cerevisiae,” Eukaryotic Cell, vol. 10, no. 3, pp. 398–411, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Kofuji, T. Ito, Y. Murata, and S. Kawashima, “Biodegradation and drug release of chitosan gel beads in subcutaneous air pouches of mice,” Biological and Pharmaceutical Bulletin, vol. 24, no. 2, pp. 205–208, 2001. View at Publisher · View at Google Scholar · View at Scopus
  25. Y. Murata, S. Toniwa, E. Miyamoto, and S. Kawashima, “Preparation of alginate gel beads containing chitosan salt and their function,” International Journal of Pharmaceutics, vol. 176, no. 2, pp. 265–268, 1999. View at Publisher · View at Google Scholar · View at Scopus
  26. J. J. Xuan, P. Balakrishnan, D. H. Oh et al., “Rheological characterization and in vivo evaluation of thermosensitive poloxamer-based hydrogel for intramuscular injection of piroxicam,” International Journal of Pharmaceutics, vol. 395, no. 1-2, pp. 317–323, 2010. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. P. Cid, V. Pedrazzi, V. P. de Sousa, and M. B. R. Pierre, “In vitro characterization of chitosan gels for buccal delivery of celecoxib: influence of a penetration enhancer,” Amercian Association of Pharmaceutical Scientists, vol. 13, no. 1, pp. 101–111, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. J. Das Neves, M. V. Da Silva, M. P. Gonçalves, M. H. Amaral, and M. F. Bahia, “Rheological properties of vaginal hydrophilic polymer gels,” Current Drug Delivery, vol. 6, no. 1, pp. 83–92, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. L. R. Gaspar and P. M. B. G. Maia Campos, “Rheological behavior and the SPF of sunscreens,” International Journal of Pharmaceutics, vol. 250, no. 1, pp. 35–44, 2003. View at Publisher · View at Google Scholar · View at Scopus
  30. A. Martin, Physical Pharmacy, Lea & Febiger, Philadelphia, Pa, USA, 4th edition, 1993.
  31. A. A and Berretta, Desenvolvimento e avaliação de uma forma farmacêutica de liberação sustentada contendo extrato padronizado de própolis para o tratamento de queimaduras [dissertação de mestrado], Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil, 2003.
  32. E. J. Ricci, M. V. L. B. Bentley, M. Farah, R. E. S. Bretas, and J. M. Marchetti, “Rheological characterization of Poloxamer 407 lidocaine hydrochloride gels,” European Journal of Pharmaceutical Sciences, vol. 17, no. 3, pp. 161–167, 2002. View at Publisher · View at Google Scholar · View at Scopus
  33. G. P. Andrews and D. S. Jones, “Rheological characterization of bioadhesive binary polymeric systems designed as platforms for drug delivery implants,” Biomacromolecules, vol. 7, no. 3, pp. 899–906, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. J. das Neves and M. F. Bahia, “Review—gels as vaginal drug delivery systems,” International Journal of Pharmaceutics, vol. 318, no. 1-2, pp. 1–14, 2006. View at Publisher · View at Google Scholar · View at Scopus
  35. A. G. Mikos and N. A. Peppas, “Measurement of the surface tension of mucin solutions,” International Journal of Pharmaceutics, vol. 53, no. 1, pp. 1–5, 1989. View at Google Scholar · View at Scopus
  36. G. A. Burdock, “Review of the biological properties and toxicity of bee propolis (propolis),” Food and Chemical Toxicology, vol. 36, no. 4, pp. 347–363, 1998. View at Publisher · View at Google Scholar · View at Scopus
  37. B. A. Rocha, P. C. P. Bueno, M. M. O. L. L. Vaz et al., “Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 670451, 11 pages, 2013. View at Publisher · View at Google Scholar
  38. M. L. Bruschi, M. L. C. Cardoso, M. B. Lucchesi, and M. P. D. Gremião, “Gelatin microparticles containing propolis obtained by spray-drying technique: preparation and characterization,” International Journal of Pharmaceutics, vol. 264, no. 1-2, pp. 45–55, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. M. L. Bruschi, E. H. G. Lara, C. H. G. Martins et al., “Preparation and antimicrobial activity of gelatin microparticles containing propolis against oral pathogens,” Drug Development and Industrial Pharmacy, vol. 32, no. 2, pp. 229–238, 2006. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Fernandes Jr., E. C. Balestrin, J. E. C. Betoni, R. De Oliveira Orsi, M. D. L. R. De Souza Da Cunha, and A. C. Montelli, “Propolis: anti-Staphylococcus aureus activity and synergism with antimicrobial drugs,” Memorias do Instituto Oswaldo Cruz, vol. 100, no. 5, pp. 563–566, 2005. View at Google Scholar · View at Scopus
  41. R. Longhini, S. M. Raksa, A. C. P. Oliveira, T. I. Svidzinski, and S. L. Franco, “Obtenção de extratos de própolis sob diferentes condições e avaliação de sua atividade antifúngica,” Revista Brasileira Farmacognosia, vol. 17, no. 3, pp. 388–395, 2007. View at Google Scholar
  42. A. C. H. F. Sawaya, A. M. Palma, F. M. Caetano et al., “Comparative study of in vitro methods used to analyse the activity of propolis extracts with different compositions against species of Candida,” Letters in Applied Microbiology, vol. 35, no. 3, pp. 203–207, 2002. View at Publisher · View at Google Scholar · View at Scopus
  43. J. Sikkema, J. A. M. De Bont, and B. Poolman, “Mechanisms of membrane toxicity of hydrocarbons,” Microbiological Reviews, vol. 59, no. 2, pp. 201–222, 1995. View at Google Scholar · View at Scopus
  44. B. F. Brehm-Stecher and E. A. Johnson, “Sensitization of Staphylococcus aureus and Escherichia coli to antibiotics by the sesquiterpenoids nerolidol, farnesol, bisabolol, and apritone,” Antimicrobial Agents and Chemotherapy, vol. 47, no. 10, pp. 3357–3360, 2003. View at Publisher · View at Google Scholar · View at Scopus
  45. J. M. Hornby, E. C. Jensen, A. D. Lisec et al., “Quorum sensing in the dimorphic fungus Candida albicans is mediated by farnesol,” Applied and Environmental Microbiology, vol. 67, no. 7, pp. 2982–2992, 2001. View at Publisher · View at Google Scholar · View at Scopus
  46. V. V. Khutoryanskiy, “Advances in mucoadhesion and mucoadhesive polymers,” Macromolecular Bioscience, vol. 11, no. 6, pp. 748–764, 2011. View at Publisher · View at Google Scholar · View at Scopus
  47. S. A. Mortazavi and J. D. Smart, “An investigation of some factors influencing the in vitro assessment of mucoadhesion,” International Journal of Pharmaceutics, vol. 116, no. 2, pp. 223–230, 1995. View at Publisher · View at Google Scholar · View at Scopus
  48. B. W. Barry, “Recent advanced in pharmaceutical rheology,” Journal of Texture Studies, vol. 1, pp. 405–430, 2007. View at Google Scholar
  49. L. Perioli, V. Ambrogi, L. Venezia, C. Pagano, M. Ricci, and C. Rossi, “Chitosan and a modified chitosan as agents to improve performances of mucoadhesive vaginal gels,” Colloids and Surfaces B, vol. 66, no. 1, pp. 141–145, 2008. View at Publisher · View at Google Scholar · View at Scopus
  50. M. R. Henzl, “Properties of sustained-release single-dose formulations for vulvovaginal candidiasis: the example of butoconazole vaginal emulsion as a drug-sparing formulation,” American Journal of Drug Delivery, vol. 3, no. 4, pp. 227–237, 2005. View at Publisher · View at Google Scholar · View at Scopus
  51. V. R. Scott, R. Boehme, and T. R. Matthews, “New class of antifungal agents: jasplakinolide, a cyclodepsipeptide from the marine sponge, Jaspis species,” Antimicrobial Agents and Chemotherapy, vol. 32, no. 8, pp. 1154–1157, 1988. View at Google Scholar · View at Scopus
  52. G. M. González, O. J. Portillo, G. I. Uscanga et al., “Therapeutic efficacy of voriconazole against a fluconazole-resistant Candida albicans isolate in a vaginal model,” Journal of Antimicrobial Chemotherapy, vol. 64, no. 3, pp. 571–573, 2009. View at Publisher · View at Google Scholar · View at Scopus
  53. D. A. Stevens, L. Calderon, M. Martinez, K. V. Clemons, S. J. Wilson, and C. P. Selitrennikoff, “Zeamatin, clotrimazole and nikkomycin Z in therapy of a Candida vaginitis model,” Journal of Antimicrobial Chemotherapy, vol. 50, no. 3, pp. 361–364, 2002. View at Google Scholar · View at Scopus
  54. Y. G. Bachhav and V. B. Patravale, “Pharmaceutical nanotechnology—microemulsion based vaginal gel of fluconazole: formulation, in vitro and in vivo evaluation,” International Journal of Pharmaceutics, vol. 365, no. 1-2, pp. 175–179, 2009. View at Publisher · View at Google Scholar · View at Scopus