Garlic-Derived S-Allylmercaptocysteine Ameliorates Nonalcoholic Fatty Liver Disease in a Rat Model through Inhibition of Apoptosis and Enhancing Autophagy

Figure 2

Addition of SAMC during the development of NAFLD reduced the hepatic apoptosis in rats. ((a)–(d)) Representative results of TUNEL assay in the rat liver sections by fast red staining ((a) control, (b) NAFLD, (c) SAMC, (d) NAFLD+SAMC) and (e) quantitative data of TUNEL assay results (a.u. = arbitrary unit). (f) Protein expression of cleaved (activated) caspase-3 was measured by Western blot and then quantified by ImageJ software. Data presented are expressed as mean ± SEM (𝑛=7), and experimental groups marked by different letters represented significant differences between groups at 𝑃<0.05 (Kruskal-Wallis test followed by Dunn’s post hoc test). Magnification: 200x. Bar: 20 microns. N + S: NAFLD + SAMC cotreatment.

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