Ethanol Extracts of Fresh Davallia formosana (WL1101) Inhibit Osteoclast Differentiation by Suppressing RANKL-Induced Nuclear Factor-κB Activation
Figure 1
Inhibition of osteoclast differentiation by WL1101 in primary cultured cells. Osteoclast precursors (bone marrow macrophages) isolated from long bone of adult male rats were incubated in the presence of mouse recombinant soluble RANKL (5 ng/mL) and murine M-CSF (20 ng/mL) with or without WL1101 for 5 days. (a) Representative images of TRAP staining showed that treatment with WL1101 exerted a concentration-dependent inhibition on osteoclastogenesis. The quantitative data are shown in (b). TRAP-positive cells containing three or more nuclei were counted. Each value represents mean ± S.E.M. (); compared with control vehicle. Scale bar: 100 μm.
