Ethanol Extracts of Fresh Davallia formosana (WL1101) Inhibit Osteoclast Differentiation by Suppressing RANKL-Induced Nuclear Factor-κB Activation
Figure 3
WL1101 inhibits RANKL-induced NF-κB activation in RAW264.7 cells. (a) RAW264.7 cells were preincubated with WL1101 (200 μg/mL) for 30 min and then administered with RANKL (10 ng/mL) at several time intervals. Western blotting showed that treatment with RANKL rapidly increased the phosphorylation of IKK, NF-κB-p65, and IκBα. Pretreatment with WL1101 ameliorated the activation of IKK, NF-κB-p65, and IκBα. (b) RAW264.7 cells were preincubated with WL1101 (200 μg/mL) for 30 min and then incubated with RANKL (10 ng/mL) for another 30 min. Cytosolic and nuclear extracts were separated by NE-PER. Western blot analysis showed that WL1101 significantly inhibited the translocation of NF-κB subunits of p50 and p65 from cytosol into nucleus. C23 (Nucleolin, NCL) was used for internal control of nuclear protein ().
