Icariin Augments Bone Formation and Reverses the Phenotypes of Osteoprotegerin-Deficient Mice through the Activation of Wnt/β-Catenin-BMP Signaling
Icariin activated canonical Wnt/β-catenin signaling in bone marrow stromal cells. Primary bone marrow stromal cells were cultured with or without Icariin (1, 10, and 50 μM) for 2 days; then the mRNA expression of Wnt ligands, β-catenin protein expression, and β-catenin signaling reporter (TOPGAL) activity were, respectively, examined. Data were expressed as mean ± SD, . 50 μM Icariin significantly increased the mRNA expression of Wnt1 (a) and Wnt3a (b) and significantly upregulated active-β-catenin protein levels (c). 50 μM Icariin significantly increased the luciferase activity of the TOPGAL reporter (d). , unpaired Student’s t-test (Icariin versus vehicle control).
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.