Icariin Augments Bone Formation and Reverses the Phenotypes of Osteoprotegerin-Deficient Mice through the Activation of Wnt/β-Catenin-BMP Signaling

Table 1

Mouse primers for real-time quantitative PCR assays.

Genes	Forward primer	Reverse Primer
𝛽-actin	TGTTACCAACTGGGACGACGACA	CTGGGTCATCTTTTCACGGT
ALP	TGACCTTCTCTCCTCCATCC	CTTCCTGGGAGTCTCATCCT
BMP2	ACTTTTCTCGTTTGTGGAGC	GAACCCAGGTGTCTCCAAGA
BMP4	GAGGAGGAGGAAGAGCAGAG	TGGGATGTTCTCCAGATGTT
RUNX2	TCCTGTAGATCCGAGCACCA	CTGCTGCTGTTGTTGCTGTT
OC	CTTGAAGACCGCCTACAAAC	GCTGCTGTGACATCCATAC
Wnt1	ACAGCGTTCATCTTCGCAATCACC	AAATCGATGTTGTCACTGCAGCCC
Wnt3a	GGCTCCTCTCGGATACCTCT	GGGCATGATCTCCACGTAGT
AXIN2	AGTCCATCTTCATTCCGCCTAGC	AAGCTGCGTCGGATACTTGCGA
DKK1	ATTCCAACGCTATCAAGAACC	CCAAGGTGCTATGATCATTACC
TCF1	CCCCGATCTCTCTGGATTTT	AAGGGGACAGGGGTAGAG
LEF1	TCATCACCTACAGCGACGAG	GAAGGTGGGGATTTCAGGAG