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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 659165, 13 pages
Research Article

Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl4-Oxidative Stress in Several Rat Tissues

1Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, 04530 México, DF, Mexico
2Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Edificio F, Segundo Piso, Laboratorio 209, 04510 México, DF, Mexico
3Servicio de Endocrinología, Instituto Nacional de Pediatría, Insurgentes Sur 3700-C, 04530 México, DF, Mexico
4Departamento de Biología Celular, Escuela Médico Militar, Universidad del Ejército y Fuerza Aérea, 11200 México, DF, Mexico
5Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México 04510 México, DF, Mexico

Received 24 October 2012; Revised 7 December 2012; Accepted 12 December 2012

Academic Editor: José Luis Ríos

Copyright © 2013 Elvia Coballase-Urrutia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was designed to test the hypothesis that the acetonic and methanolic extracts of H. inuloides prevent carbon tetrachloride-(CCl4) induced oxidative stress in vital tissues. Pretreatment with both H. inuloides extracts or quercetin attenuated the increase in serum activity of alkaline phosphatase (ALP), total bilirubin (BB), creatinine (CRE), and creatine kinase (CK), and impeded the decrease of -globulin ( -GLOB) and albumin (ALB) observed in CCl4-induced tissue injury. The protective effect was confirmed by histological analysis with hematoxylin-eosin and periodic acid/Schiff's reagent. Level of lipid peroxidation was higher in the organs of rats exposed to CCl4 than in those of the animals treated with Heterohteca extracts or quercetin, and these showed levels similar to the untreated group. Pretreatment of animals with either of the extracts or quercetin also prevented the increase of 4-hydroxynonenal and 3-nitrotyrosine. Pretreatment with the plant extracts or quercetin attenuated CCl4 toxic effects on the activity of several antioxidant enzymes. The present results strongly suggest that the chemopreventive effect of the extracts used and quercetin, against CCl4 toxicity, is associated with their antioxidant properties and corroborated previous results obtained in liver tissue.