Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 2

Effect of extracts of H. inuloides, and quercetin on the antioxidant enzymes in heart tissue of Wistar rats untreated and CCl₄ treated at dose of 1.5 mL/kg for three consecutive days.

Group	CAT k/mg protein	SOD U/mg protein	GPx U/mg protein	GR U/mg protein	GST; 𝜇mol CDNB/min/mg protein
O.O	1.66 ± 0.05	55.28 ± 2	0.036 ± 0.001	0.179 ± 0.03	0.552 ± 0.02
P.B	1.57 ± 0.01	40.32 ± 1	0.041 ± 0.005	0.174 ± 0.04	0.324 ± 0.03
Ac.E	1.31 ± 0.01	35.26 ± 3	0.033 ± 0.002	0.161 ± 0.01	0.315 ± 0.02
Me.E	1.38 ± 0.01	41.95 ± 2	0.047 ± 0.001	0.163 ± 0.03	0.513 ± 0.02
Quer	1.55 ± 0.02	38.22 ± 2	0.051 ± 0.001	0.161 ± 0.10	0.368 ± 0.02
CCl4	1.11 ± 0.01*	23.29 ± 3*	0.027 ± 0.001*	0.095 ± 0.01*	0.109 ± 0.03^●, #
CCl4 + Ac.E	1.33 ± 0.02	31.20 ± 1	0.031 ± 0.002	0.155 ± 0.06	0.369 ± 0.03
CCl4 + Me.E	1.35 ± 0.03	33.97 ± 2	0.036 ± 0.001	0.163 ± 0.07	0.569 ± 0.03
CCl4 + Quer	1.38 ± 0.04	35.14 ± 3	0.048 ± 0.001	0.156 ± 0.01	0.516 ± 0.03

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment. *𝑃<0.01 versus all groups, ^●𝑃<0.05 versus P.B, and ^#𝑃<0.01 versus O.O, P.B. Ac.E, Me.E, Quer, CCl₄ + Me.E, CCl₄ + Ac.E, CCl₄ + Quer. Each determination was performed twice, in triplicate, and the values represent the mean ± SD. 𝑛=3.