Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 3

Effect of extracts of H. inuloides and quercetin on the antioxidant enzymes in kidney tissue of Wistar rats untreated and CCl₄-treated at dose of 1.5 mL/kg for three consecutive days.

Group	CAT k/mg protein	SOD U/mg protein	GPx U/mg protein	GR U/mg protein	GST 𝜇mol conjugate CDNB/min/mg protein
O.O	0.156 ± 0.01	71.35 ± 2	0.046 ± 0.01	0.125 ± 0.01	0.331 ± 0.01
P.B	0.136 ± 0.02	74.76 ± 2	0.042 ± 0.01	0.112 ± 0.01	0.309 ± 0.01
Ac.E	0.128 ± 0.01	64.61 ± 3	0.037 ± 0.02	0.129 ± 0.01	0.321 ± 0.01
Me.E	0.144 ± 0.02	69.84 ± 1	0.043 ± 0.01	0.152 ± 0.01	0.354 ± 0.03
Quer	0.146 ± 0.01	70.70 ± 2	0.038 ± 0.02	0.147 ± 0.02	0.340 ± 0.01
CCl4	0.091 ± 0.01^●,+	42.70 ± 2*	0.018 ± 0.01*	0.088 ± 0.009^#,†	0.254 ± 0.03*
CCl4 + Ac.E	0.135 ± 0.03	54.30 ± 2	0.035 ± 0.02	0.118 ± 0.01	0.325 ± 0.01
CCl4 + Me.E	0.138 ± 0.02	57.17 ± 1	0.041 ± 0.01	0.129 ± 0.01	0.329 ± 0.03
CCl4 + Quer	0.150 ± 0.01	68.84 ± 2	0.034 ± 0.02	0.140 ± 0.01	0.339 ± 0.01

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/ 200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment. ^●𝑃<0.01 versus O.O, P.B, Me.E, Que, CCl₄ + Ac.E, CCl₄ + Me.E, CCl₄ + Quer, ⁺𝑃<0.05 versus, Ac.E, *𝑃<0.01 versus. all groups, ^#𝑃<0.01 versus O.O, Ac.E, Me.E, Que, CCl₄ + Ac.E, CCl₄ + Me.E, CCl₄ + Quer., ^†𝑃<0.05 versus P.B. Each determination was performed twice, in triplicate, and the values represent the mean ± SD. 𝑛=3.