Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 5

Effect of extracts of H. inuloides and quercetin, on the antioxidant enzymes in cerebellum tissue of Wistar rats untreated and CCl₄ treated at dose of 1.5 mL/kg for three consecutive days.

Group	CAT k/mg protein	SOD U/mg protein	GPx U/mg protein	GR U/mg protein	GST 𝜇mol CDNB/min/mg protein
O.O	0.074 ± 0.005	76.31 ± 4.0	0.040 ± 0.001	0.032 ± 0.001	0.187 ± 0.002
P.B	0.064 ± 0.001	57.07 ± 1.4	0.038 ± 0.002	0.022 ± 0.002	0.160 ± 0.003
Ac.E	0.051 ± 0.005	50.33 ± 1.7	0.030 ± 0.001	0.016 ± 0.03	0.152 ± 0.002
Me.E	0.057 ± 0.003	55.75 ± 1.4	0.034 ± 0.001	0.017 ± 0.001	0.154 ± 0.004
Quer	0.059 ± 0.003	55.10 ± 1.1	0.036 ± 0.001	0.019 ± 0.004	0.146 ± 0.004
CCl4	0.028 ± 0.002*	40.88 ± 1.8*	0.015 ± 0.001*	0.009 ± 0.003*	0.112 ± 0.005*
CCl4 + Ac.E	0.047 ± 0.002	34.40 ± 1.7	0.035 ± 0.002	0.011 ± 0.003	0.135 ± 0.004
CCl4 + Me.E	0.053 ± 0.003	37.17 ± 1.3	0.037 ± 0.001	0.017 ± 0.001	0.140 ± 0.005
CCl4 + Quer	0.060 ± 0.003	50.98 ± 1.4	0.033 ± 0.002	0.018 ± 0.003	0.143 ± 0.007

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment.*𝑃<0.01 versus all groups. Each determination was performed twice, in triplicate, and the values represent the mean ± SD. 𝑛=3.