Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 6

Effect of extracts of H. inuloides and quercetin, on the antioxidant enzymes in hemispheres tissue of Wistar rats untreated and CCl₄ treated at dose of 1.5 mL/kg for three consecutive days.

Group	CAT; k/mg protein	SOD; U/mg protein	GPx; U/mg protein	GR; U/mg protein	GST 𝜇molCDNB/min/mg protein
O.O	0.013 ± 0.001	67.13 ± 4.0	0.046 ± 0.006	0.023 ± 0.003	0.178 ± 0.002
P.B	0.012 ± 0.005	86.41 ± 0.50	0.045 ± 0.008	0.021 ± 0.002	0.150 ± 0.003
Ac.E	0.010 ± 0.003	85.24 ± 1.2	0.040 ± 0.002	0.016 ± 0.004	0.132 ± 0.009
Me.E	0.011 ± 0.002	80.83 ± 1.4	0.041 ± 0.003	0.018 ± 0.003	0.146 ± 0.005
Quer	0.011 ± 0.001	80.96 ± 1.1	0.043 ± 0.003	0.021 ± 0.003	0.151 ± 0.006
CCl4	0.003 ± 0.002^+	48.04 ± 1.8*	0.011 ± 0.001*	0.008 ± 0.002^●,‡	0.065 ± 0.008^+
CCl4 + Ac.E	0.006 ± 0.002	75.64 ± 0.04	0.039 ± 0.001	0.015 ± 0.003	0.135 ± 0.004
CCl4 + Me.E	0.010 ± 0.002	82.31 ± 1.74	0.040 ± 0.001	0.018 ± 0.002	0.140 ± 0.005
CCl4 + Quer	0.010 ± 0.003	82.85 ± 1.61	0.042 ± 0.001	0.019 ± 0.002	0.143 ± 0.007

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment. ⁺𝑃<0.05 versus all groups, *𝑃<0.01 versus all groups, ^●𝑃<0.01 versus O.O, P.B. Ac.E, Me.E, Quer, CCl₄ + Me.E, CCl₄ + Quer, ^‡𝑃<0.05 versus CCl₄ + Ac.E. Each determination was performed twice, in triplicate, and the values represent the mean ± SD. 𝑛=3.