Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 7

Effect of extracts of H. inuloides and quercetin, on the antioxidant enzymes in striatum tissue of Wistar rats untreated and CCl₄ treated at dose of 1.5 mL/kg for three consecutive days.

GROUP	CAT; k/mg protein	SOD; U/mg protein	GPx; U/mg protein	GR; U/mg protein	GST; 𝜇mol CDNB/min/mg protein
O.O	0.017 ± 0.004	67.13 ± 4.0	0.055 ± 0.006	0.033 ± 0.003	0.175 ± 0.003
P.B	0.014 ± 0.003	57.07 ± 1.4	0.053 ± 0.003	0.030 ± 0.008	0.167 ± 0.005
Ac.E	0.011 ± 0.002	50.33 ± 1.7	0.049 ± 0.007	0.027 ± 0.003	0.157 ± 0.009
Me.E	0.013 ± 0.002	55.75 ± 1.4	0.051 ± 0.001	0.030 ± 0.002	0.165 ± 0.007
Quer	0.014 ± 0.003	55.10 ± 1.1	0.055 ± 0.004	0.033 ± 0.006	0.166 ± 0.002
CCl4	0.004 ± 0.001*	28.88 ± 1.8*	0.018 ± 0.002*	0.005 ± 0.002*	0.035 ± 0.009*
CCl4 + Ac.E	0.010 ± 0.003	35.40 ± 1.2	0.043 ± 0.004	0.023 ± 0.004	0.155 ± 0.005
CCl4 + Me.E	0.012 ± 0.005	37.17 ± 1.3	0.048 ± 0.005	0.027 ± 0.005	0.161 ± 0.003
CCl4 + Quer	0.013 ± 0.004	50.98 ± 1.4	0.052 ± 0.007	0.031 ± 0.005	0.163 ± 0.002

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment. *𝑃<0.01 versus all groups. Each determination was performed twice, in triplicate, and the values represent the mean ± SD. 𝑛=3.