Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation

Table 3

Precision results for propolis water extract (PWE) and propolis ethanolic extract (PEE).

Sample	Chemical marker	Intraday repeatability (𝑛=6)			Interday repeatability (𝑛=6)			𝐹 test
		Average quantity; (mg/mL)	RSD	VAR	Average quantity; (mg/mL)	RSD	VAR
	Caffeic acid	0.342	8.25	0.00080	0.362	6.44	0.00054	0.68
	p-coumaric acid	1.078	0.44	0.00002	1.075	1.00	0.00012	5.08
PWE	trans-cinnamic acid	0.078	2.18	0.00000	0.077	0.61	0.00000	0.50
	Aromadendrin	0.097	3.38	0.00001	0.097	2.48	0.00001	1.88
	Artepillin C	4.393	1.98	0.00758	5.450	1.43	0.00603	1.26
	Caffeic acid	0.267	1.48	0.00002	0.276	0.36	0.00000	0.06
	p-coumaric acid	1.418	0.72	0.00010	1.433	0.46	0.00004	0.42
PEE	trans-cinnamic acid	0.160	2.84	0.00002	0.152	0.91	0.00000	0.16
	Aromadendrin	0.772	0.97	0.00006	0.810	1.38	0.00013	0.45
	Artepillin C	5.480	3.01	0.02727	5.135	1.77	0.00828	3.29

RSD: relative standard deviation.
VAR: variance.