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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 684071, 13 pages
http://dx.doi.org/10.1155/2013/684071
Research Article

Gan-Lu-Yin Inhibits Proliferation and Migration of Murine WEHI-3 Leukemia Cells and Tumor Growth in BALB/C Allograft Tumor Model

1School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan
2Department of Pharmacy, Da Chien General Hospital, Miaoli 36052, Taiwan
3Department of Pathology, Chung Shan Medical University, Taichung 40201, Taiwan
4Department of Pathology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
5Graduate Institute of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan

Received 21 November 2012; Accepted 4 February 2013

Academic Editor: Evan Paul Cherniack

Copyright © 2013 Fon-Chang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to explore the antitumor effect of Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, on leukemia. Ethanolic extract of GLY was applied to evaluate its regulatory mechanisms in proliferation, migration, and differentiation of WEHI-3 leukemic cells as well as antitumor effect on BALB/c mice model. The results showed that GLY markedly reduced cell proliferation and migration with induced differentiation of WEHI-3 cells. The expression level of phosphorylated FAK, Akt, ERK1/2, and Rb was decreased p21 expression while level was increased in WEHI-3 treated with GLY. The results of cell cycle analysis revealed that GLY treatment could markedly induce G1 phase arrest and decrease cell population in S phase. Moreover, experimental results demonstrated that GLY decreased the protein expression and enzyme activity of MMP-2 and MMP-9. GLY treatment also reduced WEHI-3 leukemic infiltration in liver and spleen and tumor growth in animal model. Accordingly, GLY demonstrated an inhibitory effect on tumor growth with a regulatory mechanism partially through inhibiting FAK, Akt, and ERK expression in WEHI-3 cells. GLY may provide a promising antileukemic approach in the clinical application.