The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway
Figure 4
Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on the mitochondrial transmembrane potential () in HepG2 cells. HepG2 cells were treated with ATA at the concentrations of 0, 5, and 10 μg/mL for 24 and 48 h. (a) Fluorescence changes were visualized under a fluorescence microscope after JC-1 staining. The figures shown were representative of three independent experiments. (b) The staining fluorescence was determined by flow cytometry, and the results were expressed by the ratio of green and red fluorescence. The values are presented as mean ± SD for three independent experiments. Significant differences with 0 μg/mL were designated as ***.
