The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway

Table 1

Primer sequences and amplified conditions used for RT-PCR.

Gene	Primer sequence	Product size (bp)	Annealing temperature (°C)	Cycles
β-actin	5′-CTGTCTGGCGGCACCACCAT-3′	254	54	21
	5′-GCAACTAAGTCATAGTCCGC-3′
p21	5′-AGTGGACAGCGAGCAGCTGA-3′	380	60	27
	5′-TAGAAATCTGTCATGCTGGTCTG-3′
c-Jun	5′-ATGGAAACGACCTTCTATGACG-3′	243	60	29
	5′-GTTGCTGGACTGGATTATCAGG-3′
TNF	5′-TGTAGCCCATGTTGTAGCAAAC-3′	404	54	33
	5′-ACTAGGCAAAGTCGAGATAGTC-3′
IL-8	5′-GTTTTGCCAAGGAGTGCTAAAG-3′	208	60	29
	5′-AAAACTTCTCCACAACCCTCTG-3′
PLAU	5′-TTGATTACCCAAAGAAGGAGGA-3′	390	54	33
	5′-TGGTGACTTCAGAGCCGTAGTA-3′
Cyclin E1	5′-AGTTTGCGTATGTGACAGATGG-3′	326	60	31
	5′-CTGATACCCTGAAACCTTTTGC-3′
AKT	5′-TCTACAACCAGGACCATGAGAA-3′	379	54	26
	5′-GAGTAGGAGAACTGGGGGAAGT-3′