The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway
Table 4
Genes found to be upregulated or downregulated by 3β-hydroxy-12-oleanen-27-oic acid (ATA) in HepG2 cells based on PCR array.
Functional gene groups
Symbol
UniGene
RefSeq
Description
Fold
Cell cycle control & DNA damage repair
CDKN1A (p21Waf1)
Hs.370771
NM_000389
Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
4.86
MDM2
Hs.484551
NM_002392
Mdm2 p53 binding protein homolog (mouse)
3.56
CCNE1
Hs.244723
NM_001238
Cyclin E1
−2.71
Apoptosis and cell senescence
CFLAR (CASPER)
Hs.390736
NM_003879
CASP8 and FADD-like apoptosis regulator
2.41
TNFRSF10B (DR5)
Hs.521456
NM_003842
Tumor necrosis factor receptor superfamily, member 10b
2.70
Signal transduction molecules and transcription factors
c-Jun
Hs.714791
NM_002228
C-Jun oncogene
8.55
AKT
Hs.525622
NM_005163
V-akt murine thymoma viral oncogene homolog 1
−2.29
Angiogenesis
IL-8
Hs.624
NM_000584
Interleukin 8
13.09
THBS1
Hs.164226
NM_003246
Thrombospondin 1
2.17
TNF
Hs.241570
NM_000594
Tumor necrosis factor (TNF superfamily, member 2)
8.20
ANGPT1
Hs.369675
NM_001146
Angiopoietin 1
−4.19
TEK (tie-2)
Hs.89640
NM_000459
TEK tyrosine kinase, endothelial
−7.45
SERPINB5 (maspin)
Hs.55279
NM_002639
Serpin peptidase inhibitor, clade B (ovalbumin), member 5
2.06
TGFBR1 (ALK-5)
Hs.494622
NM_004612
Transforming growth factor, beta receptor 1
−4.08
Invasion and metastasis
MMP9 (gelatinase B)
Hs.297413
NM_004994
Matrix metallopeptidase 9
−2.55
PLAU
Hs.77274
NM_002658
Plasminogen activator, urokinase
−4.88
S100A4
Hs.654444
NM_002961
S100 calcium binding protein A4
−5.70
Gene alterations after ATA-exposed HepG2 cells were analyzed using quantitative real-time PCR profiling. Only signals that differed from untreated cells by at least 2-fold were considered as significant. Changes are indicated as (+) upregulation and (−) downregulation as compared to control group.
