Figure 5: Representative images of A549 cells treated with medium alone and 19 μM of girinimbine and stained with Hoechst for nuclear, cell permeability dye, mitochondrial membrane potential dye, and cytochrome c. The images from each row are obtained from the same field of the same treatment sample. A549 produced a marked reduction in mitochondrial membrane potential and marked increases in membrane permeability and cytochrome c. Magnification 20X.