Figure 6: Quantitative analysis of girinimbine mediated apoptosis parameter. Changes in total nuclear intensity (a), cell permeability (b), mitochondrial membrane potential (c), and cytochrome c localization (d) were measured simultaneously in A549 cells. Following treatment with girinimbine, we saw statistically significant increase in total nuclear intensity, increased cell permeability, loss of mitochondrial membrane potential, and cytochrome c release from mitochondria. Each experiment was performed at least two times. Results are expressed as the means ± SD. Statistical analysis was performed with one-way analysis of variance (ANOVA) using GraphPad Prism software (version 4.0; GraphPad Software Inc., San Diego, CA). Statistical significance is expressed as ; .