Evidence-Based Complementary and Alternative Medicine

Research Article

Studies on Chromatographic Fingerprint and Fingerprinting Profile-Efficacy Relationship of Polygoni Perfoliati Herba

Table 5

MS fragmentation of the investigated compounds by HPLC-MS^a.


Peak no.	RT (min)	UV (nm)	PI/NI	Compound	References

14	11.8	330	283.17459 [M + H]⁺/—	Unknown	—
1	13.2	217, 326	330.08154 [M + NH₄]⁺/311.04016 [M − H]⁻, 623.08777 [2M − H]⁻	Unknown	—
15	15.5	325	387. 20084 [M+ H]⁺/431.19141 [M + HCOOH-H]⁻	Unknown	—
16	19.0	315	319.04379 [M + Na]⁺/295.04529 [M−H]⁻, 591.09814	Parakmerin A	[32]
17	22.4	272, 328	—/325.05576 [M − H]⁻, 651.11853 [2M − H]⁻	O-Hydroxycinnamoyl--D-glupyranoside	[34]
2	36.0	258, 355	479.08060 [M + H]⁺/477.06668 [M − H]⁻, 955.14105 [2M − H]⁻	Quercetin-3-O--D-glucuronide*	[33]
3	41.2	267	—	Quercetin-3-O--D-glucuronide methyl ester*	[7]
4	47.1	334	521.09094 [M + H]⁺/519.07715 [M − H]⁻	Ajugasterone C-20, 22-monoacetonide	[35]
5	52.6	256, 370	303.04901 [M + H]⁺/301.03473 [M − H]⁻	Quenterin*	[8]
18	53.2	255	754.25336 [M + NH₄]⁺/735.21295 [M − H]⁻, 781.21869 [M + HCOOH-H]⁻	Helonioside B	[8, 31]
6	58.9	315	798.25769 [M + NH₄]⁺/779.21729 [M − H]⁻, 825.22278 [M + HCOOH-H]⁻	Hydropiperoside	[8]
7	63.1	315	840.26898 [M + NH₄]⁺/821.22845 [M − H]⁻, 867.23395 [M + HCOOH-H]⁻	Vanicoside C	[8, 36]
10	67.2	316	974.30463 [M + NH₄]⁺/955.26428 [M − H]⁻, 477.12866	Vanicoside B	[8]
11	69.0	317	1016.31396 [M + NH₄]⁺/997.27496 [M − H]⁻, 498.13388	Vanicoside F	[8]
12	71.3	317	—	Unknown	—

RT, NI, and PI stand for retention time, negative-ion mode, and positive-ion mode, respectively.
*Identified by comparison with reference compounds.