Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 703024, 10 pages
Research Article

Antimicrobial Brazilian Propolis (EPP-AF) Containing Biocellulose Membranes as Promising Biomaterial for Skin Wound Healing

1Instituto de Química, Universidade Estadual Paulista (UNESP) CP 355, 14800-900 Araraquara, SP, Brazil
2Faculdade de Odontologia, Universidade Estadual Paulista (UNESP) Rua Humaitá 1680, 14801-903 Araraquara, SP, Brazil
3Laboratório de Pesquisa, Desenvolvimento e Inovação (P, D and I)-Apis Flora Industrial e Comercial Ltda. Rua Triunfo 945, 14020-670 Ribeirão Preto, SP, Brazil
4Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil

Received 11 January 2013; Revised 30 April 2013; Accepted 13 May 2013

Academic Editor: Zenon Czuba

Copyright © 2013 Hernane da Silva Barud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Among remarkable discoveries concerning propolis, such as antifungal, antiviral, and antioxidant activities, its anti-inflammatory, and mainly its antibacterial, properties deserve special attention when skin wound healing is concerned. Based on this and knowing the distinctive performance of bacterial (BC) membranes on wound healing, in this work it is proposed to demonstrate the potent antimicrobial activity and wound healing properties of a novel propolis containing biocellulose membrane. The obtained propolis/BC membrane was able to adsorb propolis not only on the surface, but also in its interstices demonstrated by scanning electron microscopy, X-ray diffraction, Fourier transform infrared (FT-IR) spectroscopy, and thermogravidimetric assays. Additionally, the polyphenolic compounds determination and the prominent antibacterial activity in the membrane are demonstrated to be dose dependent, supporting the possibility of obtaining propolis/BC membranes at the desired concentrations, taking into consideration its application and its skin residence time. Finally, it could be suggested that propolis/BC membrane may favor tissue repair in less time and more effectively in contaminated wounds.