ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression
Figure 7
Reintroduction of ATF3 by adeno-associated virus increased the survival rate, attenuated lung injury, and reduced serum nitrite and HBGB1 concentrations in ATF3 KO mice following LPS treatment. (a) Representative Western blots of adenovirus- (AAV-) mediated ATF3 expression in ATF3−/− mice. AAV-GFP (phosphoglycerate kinase) was used as the control vector. Data represent mean SEM (). *, . (b) Kaplan-Meyer survival rate of mice following treatment with LPS (50 mg/kg, ip). Data represent mean SEM (total 21–24). *, . (c) Plasma nitrite levels were measured at 24 hrs after LPS (5 mg/kg, ip) challenge. Data represent mean SEM (). *, #, . (d) Representative sections of the lung tissues were stained with hematoxylin and eosin. In control sections (solv denotes normal saline, without LPS challenge), normal alveoli (asterisk) and alveolar septa (arrow) with few neutrophils were observed. Scale bar, 20 μm. (e) Serum HMGB1 concentrations at 24 hrs after LPS challenge analyzed by ELISAs. Data represent mean SEM (). *, #, . Dosages of AAV, 2 108 virus.
