ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression
Figure 8
ATF3 knockout enhanced LPS-induced increase in serum and cytosolic HMGB1 concentrations in mice. (a) Serum HMGB1 concentrations after LPS challenge (5 mg/kg, ip) analyzed by ELISAs. Data represent mean SEM (). #, . (b) Representative Western blots of cytosolic HMGB1 expression in the lung tissues. The relative percentage of cytosolic HMGB1 protein normalized to actin, which was served as the internal control, was shown in (c). Data represent mean SEM (). #, . (d) Immunofluorescent localization of cytosolic HMGB-1 induced by LPS in the lung tissues of wild type and ATF3-KO mice. Scale bar, 50 m. (e) The percentage of cells expressing cytosolic HMGB1-1 was calculated from 4 fields with a total number of 200 cells examined. Data represent mean SEM (). *, .
