Research Article

Kai-Xin-San, a Chinese Herbal Decoction Containing Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria, Stimulates the Expression and Secretion of Neurotrophic Factors in Cultured Astrocytes

Figure 1

Cultures of rat astrocytes. (a) Cultured astrocytes were harvested on DIV4, 8, 12, 16, and 20, and the mRNA encoding GFAP was analyzed. Values are expressed as the percentage of increase to DIV 4 (set as 1) and in mean ± SEM, . (b) Cultured astrocytes were observed under the microscopy. Meanwhile, cultured astrocytes were stained by Cy3-anti-GFAP (shown in red) and anti-rabbit anti-oligo2 (shown in green) polyclonal antibodies observed under confocal microscopy in the presence of 0.1% Triton-X 100. Bar = 10 μm. Representative figures are showed here, .
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(a)
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(b)