Kai-Xin-San, a Chinese Herbal Decoction Containing Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria, Stimulates the Expression and Secretion of Neurotrophic Factors in Cultured Astrocytes
Figure 3
KXS stimulates the secretion of neurotrophic factor in astrocytes. (a) Different concentrations of neurotrophic factors (0–40 pg/mL) were applied onto ELISA kit, and the absorbance was detected at wavelength of 450 nm. The calibration curve of each target protein was constructed by plotting absorbance versus the concentration of each target protein. Each calibration curve was derived from 5 data points. (b) Astrocytes were treated with KXS (0.5–50 μg/mL) and Bt2-cAMP (1 mM) for 48 hours. Then, the media were collected for analysis of NGF, BDNF, and GDNF with ELISA kit. Data are expressed as percentage of increase to control (no drug treatment, that is, 0 extract here). Values are showed as the Mean ± SEM, .
