Kai-Xin-San, a Chinese Herbal Decoction Containing Ginseng Radix et Rhizoma, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria, Stimulates the Expression and Secretion of Neurotrophic Factors in Cultured Astrocytes
Figure 5
Effect of cAMP, MAPK, and estrogen signaling pathway on the expression of neurotrophic factors. Astrocytes were pre-treated with H89 (2 μM; the PKA inhibitor), U0126 (10 μM; the MEK1/2 and PKC inhibitor), and ICI 182, 780 (1 μm; the estrogen receptor inhibitor) for 3 hours and then treated with Bt2-cAMP (1 mM), TPA (100 nM), 17β-estradiol (10 nM), and DZW-652 (15 μg/mL) for 24 hours. The mRNA expression levels of neurotrophic factors in astrocytes were analyzed. Data are expressed as x Basal where the value of control is set as 1 and in mean ± SEM, where .
