Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation
Figure 2
Fermented antler extract suppresses RANKL-induced NFATc1. (a) After pretreated with vehicle or fermented antler extract (25 μg/mL) for 1 hr, BMMs were treated with RANKL (5 ng/mL) for the indicated day, and then the mRNA expression levels were analyzed by the real-time PCR. ; ; (versus “the vehicle control”). (b) The effect of fermented antler extract on the protein expression of NFATc1 was evaluated by the Western blot analysis. Actin was used as the internal control. (c) The effect of fermented antler extract on the transcriptional activity of NFATc1 was evaluated by luciferase activity assay. Activity was expressed as fold induction compared with the activity of NFAT luciferase only. pGL4 renilla luciferase activity was used to normalize the transfection efficiency and luciferase activity. (versus “the RANK”); (versus “the RANK plus RANKL”). Each experiment was performed in triplicate. Statistical differences were analyzed with the Student’s t-test and all quantitative values were presented as mean ± SD.
