Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 5

Research Article

Ethanolic Extract of Polish Propolis: Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells

Figure 5

Cytotoxic and apoptotic effects of EEP-P on LNCaP prostate cancer cells. Cells were incubated with 25–50 μg/mL EEP-P for 24–48 h. The values represent mean ± SD of three independent experiments performed in quadruplicate ( ). (a) Cytotoxic activity of EEP-P against LNCaP cells. The percentage of cell death was measured using the MTT cytotoxicity assay ( compared to control). (b) Apoptotic activity of EEP-P against LNCaP cells. Apoptotic cell death was detected by flow cytometry using annexin V-FITC staining ( compared to control).