Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 8

Research Article

Ethanolic Extract of Polish Propolis: Chemical Composition and TRAIL-R2 Death Receptor Targeting Apoptotic Activity against Prostate Cancer Cells

Figure 8

TRAIL-R2/Fc chimera block apoptosis induced by combination of EEP-P and TRAIL in LNCaP prostate cancer cells. Cells were incubated with 50 μg/mL EEP-P and/or 100 ng/mL TRAIL with or without 1 μg/mL TRAIL-R2/Fc chimera proteins for 24 h. Apoptotic cell death was detected by annexin V-FITC staining using flow cytometry. The values represent mean ± SD of three independent experiments performed in duplicate ( ) ( compared to combination EEP-P+TRAIL).