Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant
WE-CN induces the capability of stimulating allogeneic T-cell response in MLR of BMDCs. T cells were prepared from the spleens of naïve C57BL/6 mice. Purified T cells were then cocultured with PBS-, LPS- (100 ng/mL), or WE-CN- (100 μg/mL) treated BMDCs at the indicated ratio of DC : T cells for 96 hr. Cell proliferation was measured by [3H]-thymidine incorporation for 18 hr. The data shown represent the mean ± SD of samples from three wells. * compared to control. All data are representative of three independent experiments showing similar results.