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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 761454, 15 pages
Research Article

Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant

1Plant Pathology Division, Taiwan Agricultural Research Institute (TARI), Council of Agriculture (COA), Executive Yuan, Wufeng 413, Taiwan
2Graduate Institute of Immunology, National Taiwan University, Taipei 112, Taiwan
3Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu 300, Taiwan
4Institute of Biomedical Science, National Chung-Hsing University, Taichung 402, Taiwan
5Faculty of Medicine, National Yang-Ming University, Taipei 112, Taiwan
6Division of Allergy, Immunology and Rheumatology, Taichung Veterans General Hospital, Taichung 407, Taiwan
7Division of Chest Medicine, Department of Internal Medicine, Changhua Christian Hospital, Changhua 500, Taiwan
8Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
9Department of Medical Research and Education, Taichung Veterans General Hospital, Taichung 407, Taiwan

Received 8 March 2013; Revised 25 May 2013; Accepted 1 June 2013

Academic Editor: Andreas Sandner-Kiesling

Copyright © 2013 Mei-Hsing Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1: Titration experiments with purified LPS on BMDCs activation:

The result showed that DCs under the culture conditions used in our experiments failed to mature in response to LPS concentrations of <0.1 ng/mL.

Supplemental Figure 2. The stimulatory activity of WE-CN on BMDCs is not due to contamination:

Polymyxin B (5 µg/ml) did not significantly affect the CN–induced IL-12 production and upregulation of CD80 but almost completely inhibited the LPS effect in the same experiment.

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