Recombinant Protein Production of Earthworm Lumbrokinase for Potential Antithrombotic Application
Figure 1
Earthworms exhibit strong fibrinolysis. (a) An earthworm, Eisenia fetida, was collected locally (Tulsa, OK, USA) washed with deionized water and sectioned in 13 pieces. (b) The sections were applied directly to a petri dish plate containing artificial fibrin and incubated at 37°C overnight. Human tissue plasminogen (t-PA) (5 units) was used as a positive control. Deionized water and phosphate buffered saline (PBS) served as negative controls. Bigger lysis halos (3–7) indicated higher fibrinolytic activity regions. This method was described by Mihara et al. [10].
