Evaluation of the Effects of Honey on Acute-Phase Deep Burn Wounds

Figure 2

This figure shows the location of histological observations on the right sides of wounds. The zone of coagulation is located on the left side of the dashed line. On the slide of H&E staining (a), the zone of stasis is located up to 2 mm from the end of the zone of coagulation, the zone of hyperemia is located up to 3 mm from the end of the zone of coagulation, and normal skin structures are observed in the zone 4 mm outside the end of the zone of coagulation. P: panniculus carnosus muscle. On the slide of immunohistological staining for necrosis (b), we named five locations: c, the end of the zone of coagulation; s0, the start of the zone of stasis; s1, the site 1 mm from the border between the end of the zone coagulation and the start of the zone of stasis; s2, the location 2 mm from the border between the end of the zone of coagulation and the start of the zone of stasis; s3, the location 3 mm from the border between the end of the zone of coagulation and the start of the zone of stasis; and s4, the location 4 mm from the end of the zone of coagulation (the location near normal skin). We calculated the proportions of necrotic and apoptotic cells in c–s4 for each cell/tissue type (keratinocytes, appendages, cells in dermis, vascular endothelial cells, and adipocytes). On the slide of immunohistochemical staining for neutrophils (c), we identified the 5 locations, c–s4, and counted the numbers of neutrophils and macrophages in these locations per layer (dermis, hypodermis). Additionally, we calculated the overall proportion or number for the immunostaining by summing the proportions or numbers per cell/tissue type or layer for each location and calculating the averages.