Figure 4: Effect of Gambisan on lipid accumulation in 3T3-L1 cells. 3T3-L1 cells were treated with various doses (0, 100, 250, and 500 μg/mL) of Gambisan. After 10 days, cells were fixed and stained with Oil red O dye. Images of representative cells (a) scanned, (c) captured with a microscope (200x magnification), and (b) quantified by the i-solution program using a CCD camera are shown. The extent of lipid accumulation was expressed as the percentage of control. Values are expressed as mean ± SEM of five independent experiments. *** compared with control (expressed by t-test).