Figure 5: Effect of Gambisan on adipocyte-specific gene and major adipogenic transcriptional factor expression in 3T3-L1 cells. 3T3-L1 cells were treated with various doses (0, 10, 100, 250, and 500 μg/mL) of Gambisan. After 10 days, the mRNA expression levels of HSL and LPL were measured using RT-PCR, and the protein expression levels of PPARγ, C/EBPα, and SREBP-1 were measured using western blot analysis. Insets show representative RT-PCR band (a) and western blot analysis band (c) used for quantification, and bar graphs represent the band intensities of PCR products (b) and of western blots (d) adjusted by β-actin used as the internal control. Values are expressed as mean ± SEM of three independent experiments. * , ** , *** compared with control (expressed by t-test). HSL: hormone-sensitive lipase, LPL: lipoprotein lipase, RT-PCR: reverse transcription-polymerase chain reaction, PPARγ: peroxisome proliferator-activated receptor gamma, C/EBPα: CCAAT/enhancer binding protein alpha, SREBP-1: sterol regulatory element binding protein-1.