Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

Table 2

Concurrently expressed 26 genes involved in PGs metabolism of chondrocytes with Agilent Microarray Analysis.

Probe ID	Description	Genebank	Expression level			Ratio; APS-3c treatment/; Model control
			Normal control	Model control	APS-3c treatment
			IL-1β(−); APS-3c(−)	IL-1β(−); APS-3c(+)	IL-1β(+); APS-3c(+)
A_43_P12154	Aggrecan (Acan)	NM_022190	463	62	382	6.16
A_43_P16196	Xylosyltransferase I (XylT-1)	XM_341912	177	75	194	2.59
A_43_P12206	xylosyltransferase II (Xylt2)	NM_022296	429	285	301	1.06
A_44_P363522	1,4-galactosyltransferase, polypeptide 7 (GalT-I)	NM_001031661	1256	648	1370	2.11
A_44_P384798	1,3-galactosyltransferase, polypeptide 6 (GalT-II)	NM_001106699	212	125	197	1.58
A_44_P532027	Beta-1,3-glucuronyltransferase 3 (GlcAT-I)	NM_001128184	4770	2031	5461	2.69
A_42_P573352	CS N-acetylgalactosaminyltransferase 1 (Csgalnact1)	NP_001100779	1274	694	1191	1.72
A_44_P102489	CS N-acetylgalactosaminyltransferase 2 (Csgalnact2)	NM_001106616	1929	1290	2016	1.56
A_44_P412933	Chondroitin polymerizing factor (Chpf)	NM_001005906	25074	23983	26080	1.09
A_44_P243367	Chondroitin sulfate synthase 1 (Chsy1)	NM_001106268	119	55	116	2.11
A_44_P414919	Chondroitin sulfate synthase 3 (Chsy3)	XM_225912	195	226	242	1.07
A_43_P11812	Biglycan (Bgn)	NM_017087	203010	114539	193016	1.69
A_42_P647599	Decorin (Dcn)	NM_024129	135143	139790	167452	1.20
A_43_P14911	Interleukin 1 beta (Il1b)	NM_031512	1009	398	452	1.13
A_43_P15252	Collagen, type II, alpha 1 (Col2a1)	NM_012929	102073	7983	8915	1.12
A_44_P536275	ADAM metallopeptidase with thrombospondin type 1 motif, 4 (Adamts4)	NM_023959	130	190	179	0.94
A_44_P508162	ADAM metallopeptidase with thrombospondin type 1 motif, 5 (Adamts5)	NM_198761	2672	2143	2049	0.95
A_44_P178519	ADAM metallopeptidase with thrombospondin type 1 motif, 7 (Adamts7)	NM_001047101	3743	6144	4944	0.80
A_44_P387222	Matrix metallopeptidase 1a (Mmp1a)	NM_001134530	23	148	160	1.08
A_44_P996729	Matrix metallopeptidase 2 (Mmp2)	NM_031054	114913	167698	163254	0.97
A_44_P318318	Matrix metallopeptidase 3 (Mmp3)	NM_133523	64292	213227	206101	0.96
A_44_P501112	Matrix metallopeptidase 9 (Mmp9)	NM_031055	785	45585	39302	0.86
A_44_P404861	Matrix metallopeptidase 10 (Mmp10)	NM_133514	35	4867	4695	0.96
A_44_P130333	Matrix metallopeptidase 11 (Mmp11)	NM_012980	222	65	70	1.08
A_44_P555271	Matrix metallopeptidase 12 (Mmp12)	NM_053963	5424	14439	18760	1.29
A_42_P606126	Matrix metallopeptidase 13 (Mmp13)	NM_133530	33573	185998	197176	1.06

The value of expression level in normal control, model control and APS-3c treatment group is the arithmetical average of normal control (or model control and APS-3c treatment) cRNA intensity in three chips and the ratio is the geometric average of three chips.