Antioxidant Effects of Quercetin and Naringenin Are Associated with Impaired Neutrophil Microbicidal Activity

Figure 2

Effects of naringenin and quercetin on myeloperoxidase (MPO)-HOCl production. A 96-well culture plate that contained MPO (65 nM), taurine (5 mM), and H₂O₂ (50 μM) was incubated in the presence or absence of naringenin and quercetin (25 and 50 μM). The TMB solution (50 μL) was then added to the samples, and HOCl production was quantified. The positive control (i.e., without the tested substances) was used to calculate the inhibitory effect. The data are expressed as the mean ± SEM of three experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.005, and ^∗∗∗𝑃<0.001, compared with control group.