Apoptosis Induced by Tanshinone IIA and Cryptotanshinone Is Mediated by Distinct JAK/STAT3/5 and SHP1/2 Signaling in Chronic Myeloid Leukemia K562 Cells
Figure 4
Tanshinone IIA and cryptotanshinone induce apoptosis in K562 cells. (a and b) Cells were treated with tanshinone IIA (left) or cryptotanshinone (right) for 24 h. Cell lysates were prepared and subjected to Western blotting to detect expression of apoptosis-related proteins bcl-xL, mcl-1L, surviving, and cyclin D1 (a) and caspase-3, caspase-9, and PARP (b). (c) Flow cytometry analysis of sub-G1 apoptotic DNA fraction of the cells treated with tanshinone IIA or cryptotanshinone (0, 10, or 20 μM) for 24 h. After fixation in 75% ethanol, cells were stained with PI and analyzed by flow cytometry. (d) Cells were treated with tanshinone IIA or cryptotanshinone (10 or 20 μM) for 24 h. Percentage of apoptotic cells in tanshinone IIA or cryptotanshinone-treated cells by Annexin V-PI staining. Each experiment was repeated three times.
