Apoptosis Induced by Tanshinone IIA and Cryptotanshinone Is Mediated by Distinct JAK/STAT3/5 and SHP1/2 Signaling in Chronic Myeloid Leukemia K562 Cells
Figure 5
Tanshinone IIA and cryptotanshinone with imatinib synergistically inhibit the viability of K562 cells. (a) Cells were treated with various concentrations of tanshinone IIA or cryptotanshinone (0, 2.5, 5, 10, or 20 μM) for 24 h. Cell lysates were prepared and subjected to Western blotting for phospho-bcr-abl. (b) Cells were treated with tanshinone IIA (left) or cryptotanshinone (right) and/or imatinib for 24 h. Cell viability was measured by MTT assay. (c) The combination index (CI) between two drugs was determined by Chou-Talalay method and CalcuSyn software (Biosoft, Ferguson, MO, USA). (d) Cells were treated with tanshinone IIA and imatinib for 24 h at the same time. Percentage of apoptotic cells in cotreated cells by Annexin V-PI double staining.
