Intake of Blueberry Fermented by Lactobacillus plantarum Affects the Gut Microbiota of L-NAME Treated Rats

Table 1

Primers and annealing conditions used in qPCR for bacterial quantification.

Name	Sequence (5′-3′)	Target group	Amplicon size; (bp)	Annealing temperature; (°C)	Reference
Lact-16S-F; Lact-16S-R	GGAATCTTCCACAATGGACG; CGCTTTACGCCCAATAAATCCGG	Lactobacillus	217	56	[16]
Eco1457-F; Eco1652-R	CATTGACGTTACCCGCAGAAGAAGC; CTCTACGAGACTCAAGCTTGC	Enterobacteriaceae	195	60	[17]
g-Bfra-F; g-Bfra-R	ATAGCCTTTCGAAAGRAAGAT; CCAGTATCAACTGCAATTTTA	Bacteroides fragilis group	495	50	[18]
AM1-F; AM2-R	CAGCACGTGAAGGTGGGGAC; CCTTGCGGTTGGCTTCAGAT	Akkermansia	327	60	[19]
Tot-F; Tot-R	GCAGGCCTAACACATGCAAGTC; CTGCTGCCTCCCGTAGGAGT	Total bacteria	292	60	[20]